Journal: Science Advances

Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix

doi: 10.1126/sciadv.adr3530

Figure Lengend Snippet: ( A ) To visualize SMCs, transverse sections of whole cervices from days 12, 15, 17, and 19 gravid mice ( n ≥ 3) were immunofluoresently stained with specific markers for a contractile (MYH11 and SMTN) or synthetic phenotype (RBP1 and MYH10). Sections were also stained with CNN1 and α-SMA, which are markers of contractile smooth muscle and myofibroblasts. The grayscale bar (left) is a schematic representation of markers associated with a particular SMC phenotype. Green indicates positive immunostaining for each marker; blue indicates nuclei counterstained with 4′,6-diamidino-2-phenylindole (DAPI). ( B ) Control immunoglobulin G (IgG; left) was used as a control for the primary antibodies, and primary antibodies were omitted as controls for the secondary antibodies. Representative dual immunostaining of MYH10 (green) and MYH11 (red). Scale bars, 1000 μm; inset scale bars, 60 μm. Inset depicts high-power view of select regions (dashed box) of interest, which show longitudinal- and circumferentially oriented contractile SMCs, as well as synthetic SMCs in the broad subepithelial region and diffusely throughout the stroma.

Article Snippet: Sections were incubated overnight at 4°C with blocking solution containing the following primary antibodies: calponin (1:200 dilution; Abcam, ab46794), MYH10 (1:50; Cell Signaling Technologies, 3404), MYH11 (1:125; Abcam, ab224804), RBP1 (1:100, Abcam ab154881), α-SMA (1:200 dilution; Cell Signaling Technologies, 19245), or smoothelin (1:100; Novus Biologicals, NBP2-37931).

Techniques: Staining, Immunostaining, Marker, Control

Journal: European journal of medical research

Article Title: Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing macromolecular crowding in vitro.

doi: 10.1186/s40001-024-01843-2

Figure Lengend Snippet: Fig. 1 Phenotypic identification of BVSMCs (A–I). A-C α-SMA fluorescence staining of BVSMCs, α-SMA: green; nucleus: blue; C is the merged figure of A and B; D–F calponin fluorescence staining, calponin: red; nucleus: blue; F is the merged figure of D and E; G–I Smoothelin fluorescence staining, smoothelin: green; nucleus: blue; I is the merged figure of G and H; scale bar: 100 μm

Article Snippet: Antibodies used were α-SMA (1:200, NBP1-30,894, Novus Biologicals, USA), calponin (1:200, 13,938–1-AP, Proteintech, USA), and smoothelin (1:200, orb158429, Biorbyt, UK).

Techniques: Fluorescence, Staining

Journal: Journal of toxicologic pathology

Article Title: A case of hepatic leiomyosarcoma with osteosarcomatous differentiation (malignant mesenchymoma) in a dog.

doi: 10.1293/tox.2019-0065

Figure Lengend Snippet: Fig. 3. Histopathological features of leiomyosarcomatous area. Proliferation of spindle cells arranged in interlacing fascicles was observed in hematoxylin and eosin (H&E) section (A). Cytoplasm of tumor cells were immunohistochemically positive for vimentin (B), smooth muscle actin (SMA) (C) and smoothelin (D), and negative for S-100 (E) and Schwann cell (F). Bar=100 μm.

Article Snippet: For differential diagnosis, sequential sections from the hepatic lesion were immunohistochemically stained with anti-porcine vimentin mouse monoclonal antibody (diluted 1:500, clone: V9, Santa Cruz Biotechnology Inc., Dallas, TX, USA), anti-human smooth muscle actin mouse monoclonal antibody1 (diluted 1:1000, clone: 1A4, Dako, Carpinteria, CA, USA), anti-chicken smoothelin mouse monoclonal antibody (diluted 1:100, clone: R4A, Acris Antibodies, San Diego, CA, USA), anti-human S-100 rabbit monoclonal antibody (diluted 1:500, clone: EP1576Y, Abcam, Cambridge, UK), anti-human Schwann cell mouse monoclonal antibody (diluted 1:2500, clone: Schwann/2E, CosmoBio, Tokyo, Japan), and anti-cow osteocalcin mouse monoclonal antibody (diluted 1:500, clone: OC4-30, Abcam).

Techniques:

Journal: Journal of toxicologic pathology

Article Title: A case of hepatic leiomyosarcoma with osteosarcomatous differentiation (malignant mesenchymoma) in a dog.

doi: 10.1293/tox.2019-0065

Figure Lengend Snippet: Fig. 4. Histopathological features of osteosarcomatous area. Proliferative cells were intricately observed in the leiomyosarcomatous area and contained polygonal cytoplasms, around which various amounts of eosinophilic osteoid-like and bone-like matrices were observed in hematoxylin and eosin (H&E) section (A). Cytoplasm of tumor cells were immunohistochemically positive for vimentin (B) and S-100 (C), and osteoid-like and bone-like matrices around the tumor cells were positive for osteocalcin (D). On the other hand, the cytoplasm of tumor cells is negative for smooth muscle actin (SMA) (E), smoothelin (F), and Schwann cells (G). Bar=100 μm.

Article Snippet: For differential diagnosis, sequential sections from the hepatic lesion were immunohistochemically stained with anti-porcine vimentin mouse monoclonal antibody (diluted 1:500, clone: V9, Santa Cruz Biotechnology Inc., Dallas, TX, USA), anti-human smooth muscle actin mouse monoclonal antibody1 (diluted 1:1000, clone: 1A4, Dako, Carpinteria, CA, USA), anti-chicken smoothelin mouse monoclonal antibody (diluted 1:100, clone: R4A, Acris Antibodies, San Diego, CA, USA), anti-human S-100 rabbit monoclonal antibody (diluted 1:500, clone: EP1576Y, Abcam, Cambridge, UK), anti-human Schwann cell mouse monoclonal antibody (diluted 1:2500, clone: Schwann/2E, CosmoBio, Tokyo, Japan), and anti-cow osteocalcin mouse monoclonal antibody (diluted 1:500, clone: OC4-30, Abcam).

Techniques: